This kit is designed for the efficient in vitro expansion of human mononuclear cells (MNCs) from peripheral blood or umbilical cord blood into T cells. After 14 days of culture, the initial mononuclear cells can be expanded approximately 80-fold, with CD3+ T cells constituting over 90% of the total cell population. Components are clearly defined with no heterologous elements. High-quality raw materials are used, ensuring sterility, mycoplasma-free status, and low endotoxin levels.