This product is a ready-to-use, serum-free cryopreservation medium with clearly defined ingredients, specifically designed to ensure high survival rates and pluripotency maintenance of human embryonic stem cells (hESCs) during cryopreservation. Its core formulation synergistically combines precisely controlled DMSO concentration with potent protective factors to minimize ice crystal formation and cryopreservation-induced cellular damage. This ensures cells retain exceptional proliferative capacity, normal karyotypes, and intact self-renewal and tri-germ layer differentiation potential upon thawing.