This product is a well-defined, serum-free, enzyme-free medium specifically designed for the transient maintenance, gentle washing, and cell collection of embryonal carcinoma (EC) cells during passaging or experimental procedures. Its buffered formulation provides a stable physiological environment for ex vivo cells, ensuring cellular viability and membrane integrity. It effectively neutralizes and removes residual digestive enzymes such as trypsin, preventing excessive digestion damage to cells. This establishes a reliable cellular foundation for subsequent resuspension, counting, and functional assays.