SFRE199-1 Medium is a modernized culture medium developed through key optimizations and enhancements based on the classic M199 formulation. Through precise ingredient adjustments and functional module integration, it effectively strengthens support for the culture of various adherent and suspension cells, particularly primary cells and difficult-to-culture cells. Its core value lies in significantly improving initial cell attachment efficiency, maintaining high viability and functional stability during long-term culture, and ensuring high reproducibility and repeatability of culture outcomes through clearly defined chemical components and an optimized buffering system.