This product is a serum-free medium for EB66 cells, a specialized formulation designed based on the concept of chemically defined (CD) media for high-density suspension culture of avian-derived duck embryo cells (EB66) and efficient biopharmaceutical production. Its core components precisely match the exceptional proliferation and high expression characteristics exhibited by this cell line during recombinant protein and viral vaccine production (e.g., influenza vaccines, virus-like particles). By optimizing nutrient metabolic pathways specific to avian cells, incorporating high-efficiency expression promoters, and utilizing cell protectants, it significantly enhances product titer, cell viability, and process stability while completely eliminating serum batch variability and avian pathogen risks.