MDCK Serum-Free Medium is a chemically defined serum-free formulation specifically designed for canine kidney epithelial cells (MDCK). Through precise optimization of the basal medium and the addition of key components such as recombinant insulin and transferrin, it effectively supports high-density suspension culture and viral vaccine production. Its serum-free nature completely eliminates batch-to-batch variability and the risk of viral contamination, while significantly reducing the difficulty of downstream purification.