NS0 Serum-Free Medium is a chemically defined medium meticulously optimized for high-density suspension culture and efficient recombinant protein expression in NS0 mouse myeloma cells. Its core formulation builds upon the classic CDM3 basal medium architecture, further enhanced with nutrient components tailored to the unique metabolic characteristics of NS0 cells and process-adaptive modifications. This effectively supports high-viability cell growth and stable production of target products while completely eliminating batch-to-batch variability and viral contamination risks associated with serum.