This product is a serum-free medium specifically designed for visceral endoderm-like END-2 cells. Through precise component optimization and functional validation, it efficiently supports the stable proliferation and pluripotency maintenance of visceral endoderm-like END-2 cells. Its unique formulation fully activates intrinsic cellular regulatory networks, ensuring sustained secretion of essential growth factors and extracellular matrix proteins. This provides an ideal co-culture environment for precious cells such as embryonic stem cells, significantly enhancing stem cell clonogenic efficiency and long-term passage stability.