This product is an optimized, highly efficient, and specifically targeted differentiation medium designed to promote the efficient fusion and differentiation of myoblasts (such as C2C12 cell lines or primary skeletal muscle cells) into mature multinucleated myotubes. It effectively mimics the physiological environment of in vivo myofibril formation, strongly driving the expression of myogenic regulators (such as MyoD and Myogenin). This successfully induces cells to exit the cell cycle, undergo fusion, and significantly enhances the expression levels of mature skeletal muscle markers (such as myosin heavy chain).