This product is a chemically defined medium specifically designed for the efficient, directed differentiation of human embryonic stem cells into functional cardiomyocytes. Its core formulation precisely regulates key signaling pathways such as Wnt/β-catenin through temporal sequencing, effectively mimicking the microenvironment of cardiac development in vivo. This enables stable and reproducible induction of hESCs to sequentially differentiate into mesoderm, cardiac progenitor cells, and ultimately into spontaneously beating cardiomyocytes. It significantly enhances the positive expression rate of cardiomyocyte-specific markers (e.g., cTnT, α-actinin) and synchronizes beating clusters.