This product is a serum-free medium meticulously designed for the in vitro culture of human corneal limbal stem cells. Its scientifically formulated composition robustly supports efficient cell proliferation and self-renewal, stably maintaining their unique stem cell characteristics and corneal epithelial differentiation potential. It effectively prevents phenotypic loss and undesirable differentiation during culture. With clearly defined ingredients and no animal-derived components added throughout the process, it fundamentally eliminates the risk of contamination from exogenous factors.