This product is an innovative dual-stem-cell co-culture serum-free medium, meticulously designed for the synergistic growth of hematopoietic stem cells and mesenchymal stem cells. Building upon conventional single-cell culture media, it precisely balances growth signals for both cell types. This not only creates an optimal environment for hematopoietic stem cell self-renewal and multipotent differentiation, but also leverages the natural trophic factors secreted by mesenchymal stem cells to mimic the in vivo bone marrow microenvironment. Consequently, it significantly enhances the in vitro maintenance duration, expansion efficiency, and transplant potential of hematopoietic stem cells.